Jian Ma

Research Associate

jianma@uta.edu




 

 

Research Profile


My research focuses on the development of cyanide detection method and sensor for blood, water and breath air based on cobinamide-cyanide reaction. Cobinamide is the penultimate precursor in the biosynthesis of cobalamin (vitamin B12) and could bind cyanide with about 1010 greater affinity than cobalamin and thus been used for cyanide detoxification. Here, we developed that the cobinamide can be applied for the specific visual detection of micro or nano molar cyanide.

Recent topics in my research include: blood cyanide sensor, temperature dependence of Henry’s Law Constant for HCN, liquid waveguide core (LCW)-based flow injection cyanide analyzer, all of which will be explained in detail on this page.

 

Topic 1- Blood cyanide sensor


The cyanide is a potent and rapidly acting toxic agent. Death in fires is often from fume inhalation, a variety of fuel-rich fires result in large amounts of HCN as a product. HCN raises blood cyanide concentrations (BCC) in fire victims, both survivors and fatalities. However, the current methods for cyanide determination need expensive equipments or complex pretreatment procedure, which are not suitable enough for routine point of care (POC) field use. A new method is needed to give accurate BCC from smoke inhalation victims to enable clinical staff to provide appropriate managements and so give these seriously compromised patients the best chance of survival.

We measure the cyanide in blood by a cobinamide impregnated filter based sensor. 1 mL blood sample was pipetted into a screwtop microvial. 100 µL ethanol was added as defoamer. The vial was capped with the sensor cap which has built in o-ring seals to seal on top of the vial. 0.1 mL 20% (v/v) H3PO4 was added from the top tube by a 1 mL syringe. And bubble was delivered for 1 min to make acid and blood mixing. Simultaneous to the time the acid addition is begun, the DAQ signal acquisition is initiated with a 1 Hz acquisition rate. A signal LED1 (583 nm) and a reference LED2 (653 nm) are introduced by a bifurcated optical fiber. The LEDs are turned on and off alternately by the digital outputs through individual IRLI530N transistors. The software for data acquisition and LED on-off control was written in Labview 8.5.




 




Topic 2- Temperature dependence of Henry’s Law Constant for HCN


As an equilibrium partition coefficient reflecting the relative volatility/ dissolubility of a particular compound, Henry’s Law Constant (HLC) represents a key physical property with respect to the compound’s behavior and fate in the environment as well as the applicability of potential treatment methods such as air stripping of contaminated ground water. However, literature values about the HLC for HCN vary over a large range and the most widely cited temperature dependence equation is based on “personal communication” and no original data can be found.

We measure the HLC by a dynamic equilibrium system. Briefly, high purity N2 passed into a microporous polyvinylidene fluoride (PVDF) membrane tube, in which the HCN was transported across the pores in PVDF tube from the aqueous phase into the gas phase. The generated HCN was collected in a capacity fitted midget bubbler using cobinamide solution as absorber and analyzed with a photodiode array spectrophotometer based on the cobinamide-cyanide reaction. The quantitative collection of cyanide in a single bubbler was checked by placing a second bubbler in series.





Figure 2-1. Experimental arrangements.





Figure 2-2.  Vapor pressures of HCN in equilibrium with 0.03-0.35 mM HCN at 6 different temperatures.




Figure 2-3.  The Henry’s Law constant for HCN as a function of temperature.





Figure 2-4.  Henry’s Law constant for HCN at 25 °C compared with others.


 


Topic 3- LCW-based flow injection cyanide analyzer


According to Beer’s law, increasing the path length of detection cell could increase the sensitivity. Since the advent of Teflon® AF (AF is an acronym for amorphous fluoropolymer), the liquid core waveguide has become possible and popular in recent years. In this study, a 50 cm LCW was used and the chemistry was based on the established cobinamide-cyanide reaction. As the signal both from analyte and reagent could be increased, a lower cobinamide concentration (10 µM) was used and a suitable data processing was applied for increasing the S/N. The absorbance data of 583 nm, 670 nm and 531 nm were used as signal, baseline shift correction and blank correction, respectively.

The established method is fast (could be higher than 30 h-1 throughput), sensitive (LOD 0.04 µM), low reagent and sample consuming (100 µL, suitable for long time in field use), easy to couple with a diffusion scrubber for gas phase analysis.





Figure 3-1. Experimental arrangements.




 



Figure 3-2. Data processing







Figure 3-3. Calibration curves at different days






Figure 3-4. Output signal of low concentration samples



 

Education


1999.9 – 2003.6 B.S. Environmental Engineering

Wuhan Institute of Technology

2003.9 – 2008.8 Ph.D. Environmental Science

Xiamen University

 

Working Experiences


2008.9 – 2008.12 Research associate, Xiamen University

2009.2 –     Research associate, University of Texas at Arlington

 

Publications


1.     Jian Ma, Dongxing Yuan, Min Zhang, Ying Liang. Reverse flow injection analysis of nanomolar soluble reactive phosphorus in seawater with a long path length liquid waveguide capillary cell and spectrophotometric detection. Talanta, 2009, 78, 315-320. doi:10.1016/j.talanta.2008.11.017    

2.     Jian Ma, Min Zhang, Dongxing Yuan, Quanlong Li. Reverse flow injection analysis of ultra-trace nitrite in drinking water with long path length liquid waveguide capillary cell and multi-wavelength spectrophotometric detection. Chinese Journal of Analytical Chemistry (in Chinese), 2009,37(2),313

3.     Jian Ma, Dongxing Yuan, Ying Liang. Sequential injection analysis of nanomolar soluble reactive phosphorus in seawater with HLB solid phase extraction. Marine Chemistry. 2008, 111, 151-159 http://dx.doi.org/10.1016/j.marchem.2008.04.011

4.     Jian Ma, Dongxing Yuan, Ying Liang, Minhan Dai. A modifed analytical method for the shipboard determination of nanomolar cencentrations of orthophosphate in seawater. Journal of Oceanography, 2008, 64, 443-449

5.     Jian Ma, Dongxing Yuan, Bin Guan, Rong Yang, Laowei Ge. Determination of 1-hydroxyethylidene-1,1-diphosphonic acid in recycle-cooling water by ion chromatography. Chinese Journal of Chromatography (in Chinese), 2007, 25(2), 245-247.

6.     Yongming Huang, Dongxing Yuan, Jian Ma, Min Zhang, Guohe Chen. Rapid speciation of trace iron in rainwater by reverse flow injection analysis coupled to a long path length liquid waveguide capillary cell and spectrophotometric detection. Microchim Acta, 2009, 166, 221-228.

DOI: 10.1007/s00604-009-0193-8

7.     Yuanzhen Peng, Min Zhang, Jian Ma, Dongxing Yuan. Determination of trace silicate in water for semiconductor industry by flow injection automatic analysis. Chinese Journal of Analytical Chemistry (in Chinese), 2009, 37(9), 1258-1262.

8.     Zhen Zhang, Dongxing Yuan, Jian Ma, Jinshun Chen, Yufeng Hao, Qing Fu. Development of a water quality stabilizer on-line automatic monitoring system based on fluorescence tracer. Analytical Instrumentation (in Chinese), 2008, 2, 1-4.

9.     Zhengying Shen, Dongxing Yuan, Jian Ma, Meng Li. Improvement of acid orange 7 biodegradation by ultrasound assited bioreactors. Journal of Xiamen University (Natural Science) (in Chinese), 2006, 45(2), 243-247.


Patents (CN)


1.     Jian Ma, Dongxing Yuan. Shipboard analysis system for determination of ultra-trace soluble reactive phosphorus. Application No.200910110948.1; Public No. CN 101477132A

2.     Jian Ma, Dongxing Yuan, Ying Liang.A method for the determination of nanomolar soluble reactive phosphorus in seawater. Application No.200910110949.6; Public No. CN 101477039A

3.     Jian Ma, Jinshun Chen, Dongxing Yuan, Xiaolong Sun, Yufeng Hao, Rong Yang. A special instrument for the determination of active components in 1-hydroxyethylidene-1,1-diphosphonic acid product. Patent No.ZL 2007 2 0008213.4

4.     Baomin Liu, Jian Ma, Dongxing Yuan, Xiaoying Jin, Xianghai Kong, Xiaolong Sun, Yufeng Hao. A method for the determination of 1-hydroxyethylidene-1,1-diphosphonic acid. Application No.200510065453.3; Public No. CN 1670510A

5.     Zhen Zhang, Dongxing Yuan, Jian Ma, Jinshun Chen, Xiyao Liu, Yufeng Hao, Qing Fu. An on-line automatic instrument for monitoring the water quality stabilizer in recycle-cooling water system. Application No.200810071307.5; Public No. CN 101303303A

6.     Ying Liang, Dongxing Yuan, Quanlong Li, Jian Ma, Qingmei Lin. A chemiluminence method for the determination of ultra-trace soluble reactive phosphorus. Application No.200910110945.8; Public No. CN 101477057A

 

Presentations


1.     Jian Ma. Ocean instrumentation development for nanomolar level nutrient measurement in MEL/XMU. XMU-NOAA workshop, Miami, USA, June 2007.

2.     Jian Ma. Ocean instrumentation development for nanomolar level nutrient measurement in MEL/XMU. XMU-USF workshop, St Petersburg, USA, June 2007.

 

Field Experiences


1.     June 2006 (one week), Taiwan Strait Research Cruise

2.     November/December 2006 (one month), Winter Research Cruise (known as Program for Innovative Research Team in University) in South China Sea,

3.     August/September 2007 (one month), Summer Research Cruise (known as Program for Innovative Research Team in University) in South China Sea,

4.     From April 2006 to present, Xiamen Western Bay and Shenhu Bay research cruises for 12 times



AWARDS


1.      June 2008      Merit student of Xiamen University

2.      June 2008      Outstanding graduate of Xiamen University

3.      November 2007 Excellent Award of Protect the ocean and environment Photo
              Competition

4.      November 2005 Excellent Student Leaders of Xiamen University

5.      December 2004 Excellent Athlete of College of Oceanography and     
              Environmental Science of Xiamen University



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