My research focuses on the development of cyanide detection method and sensor for blood, water and breath air based on cobinamide-cyanide reaction. Cobinamide is the penultimate precursor in the biosynthesis of cobalamin (vitamin B12) and could bind cyanide with about 1010 greater affinity than cobalamin and thus been used for cyanide detoxification. Here, we developed that the cobinamide can be applied for the specific visual detection of micro or nano molar cyanide.
Recent topics in my research include: blood cyanide sensor, temperature dependence of Henry’s Law Constant for HCN, liquid waveguide core (LCW)-based flow injection cyanide analyzer, all of which will be explained in detail on this page.
The cyanide is a potent and rapidly acting toxic agent. Death in fires is often from fume inhalation, a variety of fuel-rich fires result in large amounts of HCN as a product. HCN raises blood cyanide concentrations (BCC) in fire victims, both survivors and fatalities. However, the current methods for cyanide determination need expensive equipments or complex pretreatment procedure, which are not suitable enough for routine point of care (POC) field use. A new method is needed to give accurate BCC from smoke inhalation victims to enable clinical staff to provide appropriate managements and so give these seriously compromised patients the best chance of survival.
cyanide in blood by a
cobinamide impregnated filter based sensor. 1 mL blood sample was
a screwtop microvial. 100 µL ethanol was added as defoamer. The vial
with the sensor cap which has built in o-ring seals to seal on top of
0.1 mL 20% (v/v) H3PO4 was added from the top
tube by a 1
mL syringe. And bubble was delivered for 1 min to make acid and blood
Simultaneous to the time the acid addition is begun, the DAQ signal
is initiated with a 1 Hz acquisition rate. A signal LED1 (583 nm) and a
reference LED2 (653 nm) are introduced by a bifurcated optical fiber.
are turned on and off alternately by the digital outputs through
IRLI530N transistors. The software for data acquisition and LED
on-off control was written in Labview 8.5.
of Henry’s Law Constant for HCN
As an equilibrium partition coefficient reflecting the relative volatility/ dissolubility of a particular compound, Henry’s Law Constant (HLC) represents a key physical property with respect to the compound’s behavior and fate in the environment as well as the applicability of potential treatment methods such as air stripping of contaminated ground water. However, literature values about the HLC for HCN vary over a large range and the most widely cited temperature dependence equation is based on “personal communication” and no original data can be found.
We measure the HLC by
a dynamic equilibrium system. Briefly,
high purity N2 passed into a
microporous polyvinylidene fluoride
(PVDF) membrane tube, in which the HCN was transported across the pores
tube from the aqueous phase into the gas phase. The generated HCN was
in a capacity fitted midget bubbler using cobinamide solution as
analyzed with a photodiode array spectrophotometer based on the
cobinamide-cyanide reaction. The quantitative collection of cyanide in
bubbler was checked by placing a second bubbler in series.
2-2. Vapor pressures of HCN in equilibrium with
0.03-0.35 mM HCN
at 6 different temperatures.
2-3. The Henry’s Law constant for HCN as a function
2-4. Henry’s Law
constant for HCN at 25 °C compared with others.
injection cyanide analyzer
According to Beer’s law, increasing the path length of detection cell could increase the sensitivity. Since the advent of Teflon® AF (AF is an acronym for amorphous fluoropolymer), the liquid core waveguide has become possible and popular in recent years. In this study, a 50 cm LCW was used and the chemistry was based on the established cobinamide-cyanide reaction. As the signal both from analyte and reagent could be increased, a lower cobinamide concentration (10 µM) was used and a suitable data processing was applied for increasing the S/N. The absorbance data of 583 nm, 670 nm and 531 nm were used as signal, baseline shift correction and blank correction, respectively.
method is fast (could be higher than 30 h-1
throughput), sensitive (LOD 0.04 µM), low reagent and sample consuming
suitable for long time in field use), easy to couple with a diffusion
for gas phase analysis.
Calibration curves at different days
Output signal of low concentration samples
1999.9 – 2003.6 B.S. Environmental Engineering
Wuhan Institute of Technology
2003.9 – 2008.8 Ph.D. Environmental Science
2008.9 – 2008.12 Research associate, Xiamen University
2009.2 – Research associate, University of Texas at Arlington
1. Jian Ma, Dongxing Yuan, Min Zhang, Ying Liang. Reverse flow injection analysis of nanomolar soluble reactive phosphorus in seawater with a long path length liquid waveguide capillary cell and spectrophotometric detection. Talanta, 2009, 78, 315-320. doi:10.1016/j.talanta.2008.11.017
2. Jian Ma, Min Zhang, Dongxing Yuan, Quanlong Li. Reverse flow injection analysis of ultra-trace nitrite in drinking water with long path length liquid waveguide capillary cell and multi-wavelength spectrophotometric detection. Chinese Journal of Analytical Chemistry (in Chinese), 2009,37(2),313
3. Jian Ma, Dongxing Yuan, Ying Liang. Sequential injection analysis of nanomolar soluble reactive phosphorus in seawater with HLB solid phase extraction. Marine Chemistry. 2008, 111, 151-159 http://dx.doi.org/10.1016/j.marchem.2008.04.011
4. Jian Ma, Dongxing Yuan, Ying Liang, Minhan Dai. A modifed analytical method for the shipboard determination of nanomolar cencentrations of orthophosphate in seawater. Journal of Oceanography, 2008, 64, 443-449
5. Jian Ma, Dongxing Yuan, Bin Guan, Rong Yang, Laowei Ge. Determination of 1-hydroxyethylidene-1,1-diphosphonic acid in recycle-cooling water by ion chromatography. Chinese Journal of Chromatography (in Chinese), 2007, 25(2), 245-247.
6. Yongming Huang, Dongxing Yuan, Jian Ma, Min Zhang, Guohe Chen. Rapid speciation of trace iron in rainwater by reverse flow injection analysis coupled to a long path length liquid waveguide capillary cell and spectrophotometric detection. Microchim Acta, 2009, 166, 221-228.
7. Yuanzhen Peng, Min Zhang, Jian Ma, Dongxing Yuan. Determination of trace silicate in water for semiconductor industry by flow injection automatic analysis. Chinese Journal of Analytical Chemistry (in Chinese), 2009, 37(9), 1258-1262.
8. Zhen Zhang, Dongxing Yuan, Jian Ma, Jinshun Chen, Yufeng Hao, Qing Fu. Development of a water quality stabilizer on-line automatic monitoring system based on fluorescence tracer. Analytical Instrumentation (in Chinese), 2008, 2, 1-4.
Yuan, Jian Ma, Meng Li.
acid orange 7 biodegradation by ultrasound assited bioreactors. Journal of Xiamen University (Natural
Science) (in Chinese), 2006, 45(2), 243-247.
1. Jian Ma, Dongxing Yuan. Shipboard analysis system for determination of ultra-trace soluble reactive phosphorus. Application No.200910110948.1; Public No. CN 101477132A
2. Jian Ma, Dongxing Yuan, Ying Liang.A method for the determination of nanomolar soluble reactive phosphorus in seawater. Application No.200910110949.6; Public No. CN 101477039A
3. Jian Ma, Jinshun Chen, Dongxing Yuan, Xiaolong Sun, Yufeng Hao, Rong Yang. A special instrument for the determination of active components in 1-hydroxyethylidene-1,1-diphosphonic acid product. Patent No.ZL 2007 2 0008213.4
4. Baomin Liu, Jian Ma, Dongxing Yuan, Xiaoying Jin, Xianghai Kong, Xiaolong Sun, Yufeng Hao. A method for the determination of 1-hydroxyethylidene-1,1-diphosphonic acid. Application No.200510065453.3; Public No. CN 1670510A
5. Zhen Zhang, Dongxing Yuan, Jian Ma, Jinshun Chen, Xiyao Liu, Yufeng Hao, Qing Fu. An on-line automatic instrument for monitoring the water quality stabilizer in recycle-cooling water system. Application No.200810071307.5; Public No. CN 101303303A
6. Ying Liang, Dongxing Yuan, Quanlong Li, Jian Ma, Qingmei Lin. A chemiluminence method for the determination of ultra-trace soluble reactive phosphorus. Application No.200910110945.8; Public No. CN 101477057A
1. Jian Ma. Ocean instrumentation development for nanomolar level nutrient measurement in MEL/XMU. XMU-NOAA workshop, Miami, USA, June 2007.
2. Jian Ma. Ocean instrumentation development for nanomolar level nutrient measurement in MEL/XMU. XMU-USF workshop, St Petersburg, USA, June 2007.
1. June 2006 (one week), Taiwan Strait Research Cruise
2. November/December 2006 (one month), Winter Research Cruise (known as Program for Innovative Research Team in University) in South China Sea,
3. August/September 2007 (one month), Summer Research Cruise (known as Program for Innovative Research Team in University) in South China Sea,
From April 2006 to
present, Xiamen Western Bay and Shenhu Bay research
cruises for 12 times
1. June 2008 Merit student of Xiamen University
2. June 2008 Outstanding graduate of Xiamen University
Excellent Award of Protect the ocean and environment Photo
4. November 2005 Excellent Student Leaders of Xiamen University
Excellent Athlete of College of Oceanography and